Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Fry, A. M., et al., 1998. EMBO J. 17:470. 2. Zeng, SR., et al. 2009. Mol Cell Biol. 112:1379. PubMed.
Whole cell extract from A431 cells (Lane 1) and NIH3T3 cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-γ-tubulin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
γ-tubulin is a 51 kD member of the tubulin superfamily and is conserved between yeast and mammals. This cytoskeletal protein is highly expressed in centrosomes and is thought to be involved in cytoskeletal organization and mitosis. γ-tubulin is expressed at highest levels during prophase to metaphase and can be modified by phosphorylation. This protein has been shown to interact with α-tubulin, paxillin, and RAC GTPase activated protein 1. The Poly6209 antibody has been shown to be useful for Western blotting of human and mouse γ-tubulin.
Other Names:
γ-tubulin
Structure:
Tubulin superfamily, highly conserved between yeast and mammals. Predicted molecular weight approximately 51 kD
Distribution:
Cytoskeletal protein, high expressed in centrosomes
Function:
Cytoskeletal organization, mitosis
Regulation:
Expressed at highest levels during prophase to metaphase
Modification:
Phosphorylation
Interaction:
Interacts with α-tubulin, paxillin, RAC GTPase activated protein 1
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
