Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended.
COA:
Application Notes:
The Poly6359 antibody recognizes human phospho-TSC2 (Ser664) and has been shown to be useful for immunofluorescent staining. This antibody does not detect TSC2 phosphorylated at other sites.
Application References:
1. Bartolome A, et al. 2010. Endocrinology. PubMed 2. Miyazaki M, et al. 2011. J Physiol. 589:1831. PubMed.
Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified rabbit polyclonal antibody Poly6359 against phospho-TSC2 (Ser664), followed by Cy3-conjugated Donkey anti-rabbit IgG and DAPI.
Formalin-fixed paraffin-embedded human epidermis tissue was stained with Poly6359 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Tuberous sclerosis complex (TSC) is an autosomal dominant genetic disorder that is caused by mutation in either the TSC1 (hamartin) or TSC2 (tuberin) gene. TSC1 and TSC2 proteins form a physical and functional heterodimer in vivo thatinhibits ribosomal protein S6 kinase 1 (S6K1) and activates eukaryotic initiation factor 4E-bindingprotein 1 (4E-BP1) by inhibition of the mammalian target of rapamycin (mTOR). TSC2 belongs to small GTPase protein of the Ras superfamily, contain a Rap-GAP domain. Predicted molecular weight 201 kD. Ser664 TSC2 phosphorylation leads to TSC1-TSC2 dissociation and markedly impairs TSC2 ability to inhibit mTOR signaling, cell proliferation, and oncogenic transformation. Erk (extracellular signal-regulated kinase) may contribute to tumorigenesis by phosphorylating TSC2 at specific residues, particularly S664.
Other Names:
Tuberin, Tuberous sclerosis 2 protein, TSC4
Structure:
Belongs to small GTPase protein of the Ras superfamily, contain a Rap-GAP domain. TSC2 has an NH2-terminal leucine zipper necessary for TSC1 binding and a COOH-terminal domain similar to that found in GTPase-activating proteins. Predicted molecular weight
Distribution:
Cytoplasm protein and is expressed in liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
Function:
Negatively regulates cell growth and proliferation as a TSC1-TSC2 heterodimer. TCS2 is believed to be a tumor suppressor and is able to stimulate specific GTPases.
Interaction:
14-3-3, AKT1, AKT3, ERK2, TSC1, Protein kinase B beta, Estrogen receptor alpha, SMAD3, p27Kip1, RAB5
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-TSC2 Phospho (Ser664)
Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified rabbit polyclonal antibody Poly6359 against phospho-TSC2 (Ser664), followed by Cy3-conjugated Donkey anti-rabbit IgG and DAPI.
Formalin-fixed paraffin-embedded human epidermis tissue was stained with Poly6359 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
