Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 4 μg per 1 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Murray, G. et al. 1998. Gut. 43:791. (IHC)
Western blot analysis of recombinant human TIMP-1 protein using anti-TIMP-1, clone F31P2A5.
Description:
TIMP-1 (Tissue inhibitor of metalloproteinase 1) is a glycoprotein that belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain, predicted molecular weight 23 kD. TIMP-1 forms complexes with matrix metalloproteinases (MMPs) and inhibits the proteolytic activity of these enzymes. High levels TIMP-1 protein has been found in several types of cancers including breast cancer and this increase associated with a poor patient prognosis. TIMP-1 has also been implicated in the inhibition of apoptosis, promotion of growth, and process of angiogenesis. TIMP-1 may be useful as a prognostic marker in combination with urokinase plasminogen activator (uPA) and plasminogen activator inhibitor type-1 (PAI-1). Clone F31P2A5 has been shown to be useful for western blotting, immunohistochemistry and ELISA of human and monkey TIMP-1.
Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain, predicted molecular weight 23 kD.
Distribution:
Extracellular, expressed in a number of connective tissues, plasma and amniotic fluid.
Function:
TIMP-1 forms complexes with matrix metalloproteinases (MMPs) and inhibits the proteolytic activity of these enzymes. High levels of TIMP-1 protein has been found in several types of cancers (eg. breast cancer) where the increase has been associated with a poor patient prognosis.
Interaction:
MMP-1, MMP-3, MMP-9
Antigen References:
1. Lambert, E., et al. 2004. Crit. Rev. Oncol./Hematol. 49: 187
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