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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 to 2.0 µg/ml antibody dilution for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Hela cell extracts untreated (lane 1) and treated with 10 Gy radiation (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-TIF1β Phospho (Ser473) antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Transcriptional intermediary factors (TIFs) are a group of transcriptional coactivators and corepressors that regulate gene expression by modulating chromatin structure and assembly of transcription initiation complexes. TIF1 beta is a member of the TIF1 subfamily of chromatin-associated TIFs that play a key role in many developmental and physiological processes.
Other Names:
KAP-1, KRIP-1, TRIM28
Structure:
An N-terminal RING-B box coiled coil (RBCC) domain most likely attributes to intermolecular structure, while two characteristic chromatin-targeting domains, a plextrin homology (PH) finger motif and bromodomain, are found in the C-terminus.
Distribution:
Nucleus
Function:
TIF1 beta acts as a transcriptional repressor by modulating histone deacetlyation, histone H3 Ly-9 methylation, and recruitment of HP1 proteins.
Interaction:
TIF1 beta was shown to form homo-oligomers in vitro, but does not form hetero-oligomers with TIF1 alpha or TIF1 gamma.
Antigen References:
1. Abrink , et al. 2001. Proc. Natl. Acad. Sci. 98(4):1422. 2. Change, et al. 2008. BMC Mol. Biology 9:1471.
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Purified anti-TIF1β Phospho (Ser473)
Hela cell extracts untreated (lane 1) and treated with 10 Gy radiation (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-TIF1β Phospho (Ser473) antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
