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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TIF1ß antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
TIFβ (transcription intermediary factor 1-beta) is an 88 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits setdbl to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with setdbl and cbx3 proteins. The 20A1 monoclonal antibody has been shown to recognize the human TIF1β protein and has been shown to be useful for Western blotting.
Tripartite motif family, contains three zinc-binding domains, a RING, a B-box type 1 and 2 domain, and a coiled-coil region. Predicted molecular weight approximately 110 kD
Distribution:
Nuclear, associates with specific chromatin regions
Function:
Forms a complex with KRAB-domain transcription factor and recruits setdbl to histone 3 to increase KRAB-mediated transcriptional repression
Antigen References:
1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366. 2. Schultz DC, et al. 2002. Genes Dev. 16:919. 3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859. 4. Friedman JR, et al. 1996. Genes Dev. 10:2067.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-TIF1β
HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TIF1ß antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
