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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5-1 μg per ml antibody dilution buffer. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1.Zhang Z, et al. 2007. Blood 109:4328. (Block) 2.Gorman CL, et al. 2008. J. Immunol. 180:1060. 3.Jenson WA, et al. 1997. Eur J. Immunol. 27:707. 4. Mao H, et al. 2010. J. Virol. 84:4148. PubMed
This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
Jurkat (1) and Raiji (2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with purified anti-CD247 (TCR zeta, CD3 zeta) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
The invariant TCR zeta chain is a member of the CD3 complex associated with the clonotypic alpha/beta TCR hetrodimer. The disulfide-linked TCRzeta homodimers transmit signals following TCR ligation. Loss of TCR zeta expression has been reported in a diverse range of disease states, including autoimmune diseases, many neoplastic conditions and chronic infections, such as tuberculosis and leprosy.
Other Names:
CD247, CD3zeta
Structure:
163 amino acid long protein with a short of extracellular domain of only nine amino acids conserved between mouse and human. There are 6-7 intracellular tyrosines that are potential phosphorylation sites.
Distribution:
T cells and NKT cells. Reported also positive on CD16+ human NK cells.
Function:
Mediating signal transduction after TCR receptor engagement.
Interaction:
ZAP70, TCR alpha/beta
Antigen References:
1.Weissman A, et al. 1988. P. Natl. Acad. Sci. USA 85:9709
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-CD247 (TCRζ, CD3ζ)
Jurkat (1) and Raiji (2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with purified anti-CD247 (TCR zeta, CD3 zeta) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
FITC anti-CD247 (TCRζ, CD3ζ)
Human peripheral blood lymphocytes surface stained with CD3 (UCHT1) PE then intracellular stained with 6B10.2 FITC
