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Purified anti-TCP-1β Antibody
Purified anti-TCP-1β Antibody
635502 100 µg £125     

Product Details

Clone: F39P7F11
Isotype: Mouse IgG2a, κ
Reactivity: Human, Mouse, Rat, Frog
Immunogen: Ovalbumin-conjugated synthetic Peptide RKRVPDHHPC
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: This antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted at 4°C.
Application: WB-Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-10 μg per 5 ml antibody dilution buffer for each mini-gel.  It is recommended that the reagent be titrated for optimal performance for each application.

COA:
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HepG2 cell extracts were resolved
HepG2 cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal TCP-1β antibody (clone F39P7F11). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.


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Description:

TCP-1β (Chaperonin containing T complex polypeptide 1, subunit 2) belongs to the TCP-1 chaperonin family, predicted molecular weight 57 kD. Chaperonin-containing TCP-1 is a large multisubunit complex of 800-900 kD, a protein complex considered to be the cytosolic homologue of mitochondrial HSP60. TCP-1β has been shown to interact with β-actin, 14-3-3, SUMO4, TCP-1α, β-Tubulin, α-Tubulin and CCT8. In vitro, TCP-1βmediates the ATP-dependent folding of tubulin, actin, and α-transducin. This protein is also essential for cyclin E maturation and accumulation.Clone F39P7F11has been shown to be useful for western blotting and immunohistochemistry of human, mouse, rat and Frog TCP-1β.

Other Names: CCT beta, CCTB, T-complex protein 1 subunit beta, TCP1 beta
Structure: Belongs to the TCP-1 chaperonin family, predicted molecular weight 57 kD.
Distribution: Cytoplasm
Function: In vitro, TCP-1β mediates the ATP-dependent folding of tubulin, actin, and α-transducin. This protein is also essential for cyclin E maturation and accumulation.
Interaction: β-actin, 14-3-3γ, SUMO4, TCP-1α, β-Tubulin, α-tubulin, CCT8
Antigen
References:
1. Yaffe MB, et al. 1992. Nature 358:245
GeneID: 21454
Latest Publications: View the latest TCP-1 beta articles on HighwirePress.com
UniProt: View information about TCP-1 beta on UniProt.org
Keywords: Purified anti-TCP-1β, F39P7F11, Purified, CCT beta, CCTB, T-complex protein 1 subunit beta, TCP1 beta , Human, Mouse, Rat, Frog, Immunology, Antibodies
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  • Purified anti-TCP-1β
    HepG2 cell extracts were resolved

    HepG2 cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal TCP-1β antibody (clone F39P7F11). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.

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