This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation:
This antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
Upon receipt, store undiluted at 4°C.
Application:
WB-Quality Tested
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-10 μg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Western blot analysis of extract from HepG2 cells using anti-TCP-1β, clone F39P7F11.
Description:
TCP-1β (Chaperonin containing T complex polypeptide 1, subunit 2) belongs to the TCP-1 chaperonin family, predicted molecular weight 57 kD. Chaperonin-containing TCP-1 is a large multisubunit complex of 800-900 kDa, a protein complex considered to be the cytosolic homologue of mitochondrial HSP60. TCP-1β has been shown to interact with β-actin, 14-3-3g, SUMO4, TCP-1α, β-Tubulin, α-Tubulin and CCT8. In vitro, TCP-1βmediates the ATP-dependent folding of tubulin, actin, and α-transducin. This protein is also essential for cyclin E maturation and accumulation.Clone F39P7F11has been shown to be useful for western blotting and immunohistochemistry of human, mouse, rat and Frog TCP-1β.?
Other Names:
CCT beta, CCTB, T-complex protein 1 subunit beta, TCP1 beta
Structure:
Belongs to the TCP-1 chaperonin family, predicted molecular weight 57 kD.
Distribution:
Cytoplasm
Function:
In vitro, TCP-1β mediates the ATP-dependent folding of tubulin, actin, and α-transducin. This protein is also essential for cyclin E maturation and accumulation.
