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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Clone 9C5 reacts with both TRAcP 5a and 5b isoforms.2 Additional reported applications (for the relevant formats) include: immunohistochemistry of formalin-fixed paraffin-embedded sections1.
Application References:
1. Janckila AJ, et al. 1995. Blood 85:2839. (IHC) 2. Janckila AJ, et al. 2005. J. Leukocyte Biol. 77:209.
Western blot analysis of recombinant human TRACP/PAP/ACP5 protein (lane 1) and PBMC (lane 2) using anti-TRACP antibody, clone 9C5.
Serum tartrate-resistant acid phosphatase exists as two isoforms (TRAcP5a and 5b). They are derived by differential-posttranslational processing of a common gene. Serum TRAcP5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis. Serum TRAcP5b is produced from osteoclasts and elevated during bone resorption. In addition, this antibody labels hairy leukemia cells with high degree of sensitivity and specificity.
In serum, TRAcP exists as two related glycoprotein isoforms derived by differential processing of a single gene product. Mature human TRAcP 5a isoform is a continuous polypeptide of approximately 300 amino acids with Mw of around 33 kD. TRAcP5b contain
Distribution:
TRAcP is expressed intracellularly at high levels in cells of monocytic lineage, including osteoclasts, macrophages and dendritic cells.
Antigen References:
1. Janckila AJ, et al. 1995. Blood 85:2839. 2. Janckila AJ, et al. 1996. J. of Histochem. and Cytochem. 44:235. 3. Janckila AJ, et al. 2005. J. Leukocyte Biol. 77:209.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
