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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
MCF7 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-TAF250 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. In addition to the TAF250 specific band, this polyclonal antibody detects an unknown protein with a molecular weight of approximately 50 kD.
TAF250 (also known as TAF1 RNA polymerase II, TATA box binding protein associated factor 250 kD, UBF initiation factor TFIID 250 kD subunit) is a 210-250 kD member of the bipartite protein kinase family containing protein kinase domains. There are two reported isoforms of this ubiquitously expressed nuclear protein. Highest expression of TAF250 has been observed in thymus, glioblastoma, and normal endometrium at mid-secretory phase. TAF250 functions as acetyltransferase and ubiquitin activating/conjugating enzyme. This protein is thought to play an essential role in TFIID assembly by interacting with TBP and other TAFs, linking cell cycle and transcriptional control. TAF250 is essential for the progression of G1 phase of the cell cycle and has been shown to stimulate ribosomal DNA transcription by binding to the activator UBF. TAF250 is modified by phosphorylation (casein kinase II). TAF250 is the largest subunit of TFIID and has been shown to interact with TBP and other TAF proteins, and UBF. The Poly6105 antibody recognizes both isoforms of human TAF250 and has been shown to be useful for Western blotting.
Other Names:
TAF1 RNA polymerase II, TATA box binding protein associated factor 250 kD, UBF initiation factor TFIID 250 kD subunit
Structure:
Bipartite protein kinase family, protein kinase domains, two isoforms, 210-250 kD
Distribution:
Nuclear, ubiquitously expressed. Highest expression in thymus, glioblastoma, normal endometrium at mid-secretory phase
Function:
Acetyltransferase activity; ubiquitin activating/conjugating enzyme. Thought to play an essential role in TFIID assembly by interacting with TBP and other TAFs, links cell cycle and transcriptional control. Essential for progression of G1 phase of cell cy
Regulation:
Phosphorylated by casein kinase II
Modification:
Phosphorylation
Interaction:
Largest subunit of TFIID, interacts with TBP and other TAF proteins, binds to UBF
Antigen References:
1. Ruppert S, et al. 1993. Nature 362:175. 2. Sekiguchi T, et al. 1991. Mol. Cell. Biol. 11:3317. 3. Martel L, et al. 2002. Mol. Cell. Biol. 22:2142.
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Purified anti-TAF250
MCF7 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-TAF250 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. In addition to the TAF250 specific band, this polyclonal antibody detects an unknown protein with a molecular weight of approximately 50 kD.
