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Each lot of this antibody is quality control tested by Western blotting and intracelluar immunofluorescent staining with flow cytometric analysis. For Western blotting, the suggested use is 1 to 2 ug per ml. For immunofluorescent staining, the suggested use of this reagent is 1.0 µg per million cells in a staining volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
Human Th1 (left) and mouse Th1 (right) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with purified 4B10. Proteins were visualized using a HRP goat anti-mouse secondary antibody and a chemiluminescent system.
T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
Other Names:
T box expressed in T cells, T box 21, TBLYM
Structure:
T-box transcription factor, approximately 58 kD.
Distribution:
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function:
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Antigen References:
1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.
Purified anti-T-bet, 4B10, Purified, T box expressed in T cells, T box 21, TBLYM , Human, Mouse, Western Blotting, Intracellular Staining for Flow Cytometry, Immunofluorescence microscopy, Immunoprecipitation, Immunology, Antibodies
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-T-bet
Human peripheral blood lymphocytes surface stained with CD3 PE then intracellularly stained with T-bet (clone 4B10) APC (top) or mouse IgG1 APC isotype control (bottom).
Purified anti-T-bet
Human Th1 (left) and mouse Th1 (right) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with purified 4B10. Proteins were visualized using a HRP goat anti-mouse secondary antibody and a chemiluminescent system.
Alexa Fluor® 647 anti-T-bet
PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellularly stained with IFN-γ (4S.B3) FITC and 4B10 Alexa Fluor® 647
PerCP/Cy5.5 anti-T-bet
PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellularly stained with CD3 PE (UCHT1) and 4B10 PerCP/Cy5.5
Pacific Blue™ anti-T-bet
Human peripheral blood lymphocytes surface stained with CD3 (UCHT1) FITC and then intracellularly stained with 4B10 Pacific Blue™
PE anti-T-bet
Human peripheral blood lymphocytes surface stained with CD3 (UCHT1) FITC then intracellular stained with 4B10 PE (top) or mIgG1,k PE isotype control (bottom)
Brilliant Violet 711™ anti-T-bet
Human peripheral blood mononuclear cells were surface stained with CD3 PE, and then intracellularly stained with T-bet (clone 4B10) Brilliant Violet 711™ (top) or mouse IgG1, κ Brilliant Violet 711™ (bottom).
FITC anti-T-bet
Human peripheral blood lymphocytes intracellularly stained with 4B10 FITC (top) or mouse IgG1,k isotype control (bottom) and CD3 APC
Brilliant Violet 421™ anti-T-bet
Human peripheral blood lymphocytes were surface stained with CD3 APC and then intracellularly stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ (bottom).
Brilliant Violet 605™ anti-T-bet
Human peripheral blood lymphocytes were surface stained with CD3 PE, and then intracellularly stained with T-bet (clone 4B10) Brilliant Violet 605™.
PE/Cy7 anti-T-bet
Human peripheral blood lymphocytes were surface stained with CD3 FITC, and then intracellularly stained with T-bet (clone 4B10) PE/Cy7 (top) or mouse IgG1, κ PE/Cy7 (bottom).
