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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µl per 5 ml. For IHC, use a 10 µg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation.
Application References:
1. Arudchandran R, et al. 2000. J. Exp. Med. 191:47. (IP WB) 2. Swann PG, et al. 1999. J. Biol. Chem. 33:23068. (IP) 3. Inoue M, et al. 201. J. Immunol. 186:18. PubMed
Formalin-fixed paraffin-embedded human tonsil tissue was stained with SYK-01 at 10 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Western blot analysis of extracts from Human PBMC, using anti-Syk, Clone SYK-01.
Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites. The SYK-01 monoclonal antibody (raised against amino acids 5-360 of human Syk) recognizes human, mouse, and rat and Syk and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.
Other Names:
Spleen tyrosine kinase, protein tyrosine kinase SYK
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Syk
Formalin-fixed paraffin-embedded human tonsil tissue was stained with SYK-01 at 10 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Western blot analysis of extracts from Human PBMC, using anti-Syk, Clone SYK-01.
