Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µl per 5 ml. For IHC, use a 10 µg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation.
Application References:
1. Arudchandran, R., et al., 2000. J. Exp. Med. 191:47. 2. Swann, P.G., et al., 1999. J. Biol. Chem. 33:23068.
Formalin-fixed paraffin-embedded human tonsil tissue was stained with SYK-01 at 10 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Western blot analysis of extracts from Human PBMC, using anti-Syk, Clone SYK-01.
Description:
Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites. The SYK-01 monoclonal antibody (raised against amino acids 5-360 of human Syk) recognizes human, mouse, and rat and Syk and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.
Other Names:
Spleen tyrosine kinase, protein tyrosine kinase SYK
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