This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
This antibody is not suitable for immunoprecipitation.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-survivin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
Survivin (also known as baculoviral IAP repeat-containing protein (BIRC5)) is a member of the IAP family containing a BIR domain. This protein has two reported isoforms α and β, with reported molecular weights of approximately 16.5 kD and 18.6 kD, respectively. Survivin can form dimers and is distributed at the microtubules of the mitotic spindle. Survivin plays a possible role in neoplasia and is expressed in embryonic tissues and the majority of human cancers, but not in most normal adult tissues. Survivin may counteract default induction of apoptosis in G2/M phase, and inhibits caspase-3 and caspase-7 activation. Survivin is downregulated by p53 and stabilized by phosphorylation. Survivin is phosphorylated by cdc2. Survivin has been shown to interact with tubulin and HBXIP. The Poly6147 antibody recognizes the C-terminal region of human survivin and has been shown to be useful for Western blotting.
Other Names:
Baculoviral IAP repeat-containing 5 (BIRC5)
Structure:
IAP family, BIR domain, dimer, two isoforms α and β, approximately 16.5 kD and 18.6 kD, respectively
Distribution:
Microtubules of mitotic spindle
Function:
Possible role in neoplasia, expressed in embryonic tissues majority of human cancers, but not in most normal adult tissues. May counteract default induction of apoptosis in G2/M phase, inhibits caspase-3, caspase-7
Regulation:
Downregulation by p53, stabilized by phosphorylation
Modification:
Phosphorylation by cdc2
Interaction:
Tubulin, HBXIP
Antigen References:
1. Li, F., et al., 1998. Nature. 396:580. 2. Chantalat, L., et al., 2000. Mol. Cell. 6:183. 3. Wall, N., et al., 2003. Cancer Res. 63:230. 4. Lens, S., et al., 2003. EMBO J. 22:2934.
