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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-STAT3 antibody (clone Poly6246). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence.
STAT 3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to cytokine receptor-associated kinase activity. Phosphorylation of STAT 3 induces nuclear translocation to activate transcription. STAT3 is expressed in brain, heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. STAT 3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT 3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT 3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT 3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by Rac1 protein. STAT 3 interacts with Rac1, STAT 1, and NCOA1 proteins. The Poly6246 antibody recognizes the C-terminal region of human, mouse, and rat STAT3 and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry. The Poly6246 antibody does not react with STAT3b.
Other Names:
Signal transducer and activator of transcription 3
Structure:
STAT family, hetero-dimers, two isoforms; 88 kD
Distribution:
Translocates into nucleus after phosphorylation. Expressed in brain, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas
Function:
Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to nucleus to act as transcriptional activator. Activates a wide variety of genes involved in cell growth and apoptosis, involved in gp130-related signaling. Bin
Regulation:
Phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, BMP2. Activity regulated by Rac1, specific inhibitors PIAS3, GRIM-19
Modification:
Phosphorylation
Interaction:
Interacts with Rac1, associates with STAT1, NCOA1
Antigen References:
1. Lufei C, et al. 2003. EMBO J. 22:1325. 2. Deo D, et al. 2002. J. Biol. Chem. 277:21237. 3. Pfeffer L, et al. 1997. Science 276:1418. 4. Akira S, et al. 1994. Cell 77:63.
Purified anti-STAT3, Poly6246, Purified, Signal transducer and activator of transcription 3, Human, Mouse, rat (does not react with STAT3b), Immunology, Antibodies
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Purified anti-STAT3
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-STAT3 antibody (clone Poly6246). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence.
