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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 2-4 µg/ml working concentration of antibody in 5% BSA for each mini-gel. For IHC, use a 15 µg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Formalin-fixed paraffin-embedded human tonsil tissue was stained with SAT-84 at 15 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse monoclonal antibody against STAT1. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
STAT1, also known as signal transduction and activator of transcription-1, is a ubiquitously expressed cytoplasmic protein that becomes phosphorylated in response to cytokine signaling (interferon α, interferon γ, EGF, PDGF, and IL-6) by receptor-associated kinases and translocates to the nucleus to act as a transcriptional activator. Two isoforms of STAT1 exist as a result of alternative RNA processing with apparent molecular weights of 88 and 91 kD. STAT1 has been shown to interact with a number of proteins including STAT3, FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27 receptor, IL-2R β and γ, IFNαRβ, STAT2, c-Src, and many others. STAT1 knockout mice are highly sensitive to infection with microbial infections and viruses. The SAT-84 monoclonal antibody recognizes human STAT1 and has been shown to be useful for Western blotting.
Other Names:
Signal transduction and activator of transcription-1, STAT-1
Structure:
STAT family, homo- and heterodimers, two isoforms from differentially processed RNA, 84 and 91 kD
Distribution:
Ubiquitous cytoplasmic expression
Function:
Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to nucleus to act as transcriptional activator. Mediates responses to interferon α, interferon γ, EGF, PDGF, and IL-6. Knock-out mice are highly sens
Modification:
Methylation, phosphorylation (serine and tyrosine), sumoylation
Interaction:
STAT3, FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27 receptor, IL-2R β and γ, IFNαRβ, STAT2, c-Src, many others
Antigen References:
1. Durbin JE, et al. 1996. Cell 8:443. 2. Darnell JE, Jr, et al. 1994. Science 264:1415. 3. Chen X, et al. 1998. Cell 93:827. 4. Ramana CV, et al. 2000. Oncogene 19:2619.
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Purified anti-STAT1
Formalin-fixed paraffin-embedded human tonsil tissue was stained with SAT-84 at 15 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse monoclonal antibody against STAT1. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
