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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
MOLT-4 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-Stat 5b antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
STAT 5b is a 90 kD member of the STAT (signal transducer and activator of transcription) protein family that is phosphorylated in response to cytokine receptor-associated kinase activity. Phosphorylation of STAT 5b induces nuclear translocation to activate transcription. STAT 5b is activated by IL-2, IL-4, CSF-1, and other growth hormones and is involved in diverse biological processes including TCR signaling, apoptosis, sexual dimorphism of liver gene expression and adult mammary gland development. STAT 5b is fused with retinoic acid receptor-α in some acute promyelocytic leukemias. STAT 5b is highly expressed in bulk germ cell seminoma and normal endometrium (late proliferative phase) and weakly expressed in fetal liver, testis, brain, human placenta, and muscle. STAT 5b has been shown to interact with the proteins nr3c1 and nmi. The Poly6101 antibody recognizes the N-terminal region of human STAT5b and has been shown to be useful for Western blotting.
Other Names:
Signal transducer and activator of transcription 5B
Structure:
STAT family, hetero-dimers; 90 kD
Distribution:
Translocates into nucleus after phosphorylation. Expressed in bulk germ cell seminoma, normal endometrium (late proliferative phase), weak expression in fetal liver, testis, brain, human placenta, muscle
Function:
Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to nucleus to act as transcriptional activator. Involved in diverse biological processes including TCR signaling, apoptosis, sexual dimorphism of liver gene expr
Regulation:
Activated by IL-2, IL-4, CSF1, and other growth hormones
Modification:
Phosphorylation
Antigen References:
1. Lin J, et al. 1996. J. Biol. Chem. 271:10738. 2. Lischke A, et al. 1998. J. Biol. Chem. 273:31222. 3. Welte T, et al. 1999. Science 283:222.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-STAT 5b
MOLT-4 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-Stat 5b antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
