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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Tan B C-M, et al. 2006. EMBO doi:10.1038/sj.emboj.7601271
293E cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-SSRP1 Antibody (clone 3E4). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 3E4 monoclonal antibody recognizes human SSRP1 and has been shown to be useful for Western blotting.
Other Names:
Structure specific recognition protein 1 (SSRP1), Facilitates Chromatin Transcription 80 kD subunit, FACTp80, Recombination signal sequence recognition protein
Structure:
SSRP family, HMG box domain; 81 kD
Distribution:
Nuclear
Function:
Interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation, binds to dsDNA. Modified by cisplatin, may protect DNA from repair and block DNA replication
Interaction:
Component of FACT ATP-dependent chromatin remodeling complex, Spt16/Cdc68 in FACT complex, H2A/H2B dimers
Antigen References:
1. LeRoy G, et al. 1998. Science 282:1836. 2. Orphanides G, et al. 1998. Cell 92:105. 3. Orphanides G, et al. 1999. Nature 400:284.
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Purified anti-SSRP1
293E cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-SSRP1 Antibody (clone 3E4). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
