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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Tan BC, et al. 2006. EMBO 25:3975. (ChIP)
Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-SSRP1 antibody. Proteins were detected using a goat anti-mouse Ig secondary conjugated to HRP and a chemiluminescence detection system.
SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT complex is composed of the SUPT16H (FACT140) and the SSRP1 FACTp80 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 10D1 monoclonal antibody recognizes the human SSRP1 protein and has been shown to be useful for Western blotting.
Other Names:
Structure specific recognition protein 1 (SSRP1), Facilitates Chromatin Transcription 80 kD subunit, FACTp80, Recombination signal sequence recognition protein< FACT 80 kD subunit, FACTp80, Chromatin-specific transcription elongation factor 80 kD subu
Structure:
SSRP family, HMG box domain; 81 kD
Distribution:
Nuclear
Function:
Interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation, binds to dsDNA. Modified by cisplatin, may protect DNA from repair and block DNA replication
Interaction:
Component of FACT ATP-dependent chromatin remodeling complex, SUPT16H/Cdc68 in FACT complex, H2A/H2B dimers
Antigen References:
1. LeRoy G, et al. 1998. Science 282:1836. 2. Orphanides G, et al. 1998. Cell 92:105. 3. Orphanides G, et al. 1999. Nature 400:284.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-SSRP1
Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-SSRP1 antibody. Proteins were detected using a goat anti-mouse Ig secondary conjugated to HRP and a chemiluminescence detection system.
