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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Tan B C-M, et al. 2006. EMBO doi:10.1038/sj.emboj.7601271
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Spt16 (Clone 8D2) antibody. Proteins were visualized using a donkey anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
Spt16 is a 120-140 kD nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. The 8D2 monoclonal antibody recognizes human Spt16 and has been shown to be useful for Western blotting.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Spt16 (FACT140 complex)
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Spt16 (Clone 8D2) antibody. Proteins were visualized using a donkey anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
