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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each minigel. For immunofluorescent staining applications, a dilution range of 1:100 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Kawamata M, et al. 2010. Proc Natl Acad Sci USA. 107:14223. PubMed
SOX2 recombinant protein (Lane 1) and mouse stem cell line F9 extracts (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose membrane, and probed with rabbit polyclonal antibody raised against C-terminal region of SOX2. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and chemiluminescence detection.
This rabbit polyclonal antibody recognizes human SOX2 also known as SRY (sex determining region Y)-box 2 and ANOP3. SOX2 is a nucleus protein, predicted molecular weight 34 kD. SOX2 belongs to the SOX (SRY-box containing gene) gene family which encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate. Defects in SOX2 are a cause of true or primary anophthalmos, also called anophthalmia or ANOP3. Anophthalmos is a developmental defect characterized by complete absence of the eyes (rare) or by the presence of vestigial eyes. The poly6309 antibody has been shown to be useful for Western blotting.
Other Names:
SRY (sex determining region Y)-box 2, ANOP3, SOX-2
Structure:
Belongs to the SOX (SRY-box containing gene) gene family, predicted molecular weight 34 kD.
Distribution:
Nucleus protein
Function:
The SOX gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate.
Interaction:
Interacts with OCT4, OCT1, FTZ1, and Fibroblast growth factor 4 proteins.
Antigen References:
1. Stevanovic M, et al. 1994. Mammalian Genome 5:640. 2. Okubo T, et al. 2006. Genes Dev. 20:2654. 3. Ragge NK, et al. 2005. Am. J. Med. Genet. 135A:1.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-SOX2 (COOH terminus)
SOX2 recombinant protein (Lane 1) and mouse stem cell line F9 extracts (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose membrane, and probed with rabbit polyclonal antibody raised against C-terminal region of SOX2. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and chemiluminescence detection.
