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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
MCF7 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-SOCS6 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
The SOCS6 (suppressor of cytokine signaling-6) protein is a 59 kD cytoplasmic protein that contains a SOCS box and an SH2 domain. SOCS6 has been implicated as an inhibitor of IR signaling and has been shown to mediate cytokine-induced insulin resistance. SOCS6 is induced by erythropoietin and GM-CSF. The SOCS6 protein has been shown to interact with elongins B and C, and the insulin receptor. This protein forms a complex with IRS-4, IRS-2, and PI3K. The Poly6155 antibody recognizes the N-terminal region of human SOCS6 and has been shown to be useful for Western blotting.
Other Names:
Suppressor of cytokine signaling-6
Structure:
SSI or SOCS family, SH2 domain, SOCS box; 59 kD
Distribution:
Cytoplasm
Function:
May be inhibitor of IR signaling, mediate cytokine-induced insulin resistance
Regulation:
Induced by erythropoietin, GM-CSF
Interaction:
Elongins B, C, insulin receptor; complex of IRS-4, IRS-2, p85 regulatory subunit of PI3K
Antigen References:
1. Masuhara M, et al. 1997. Biochem. Biophys. Res. Commun. 239:439. 2. Mooney RA, et al. 2001. J. Biol. Chem. 276:25889. 3. Krebs DL, et al. 2002. Mol Cell Biol. 22:4567.
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Purified anti-SOCS6
MCF7 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-SOCS6 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
