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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 μg per 5 ml antibody dilution buffer (1:500 dilution) for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Persico M, et al. 2008. Gut. 57:507. PubMed 2. Platten M, et al. 2009. P. Natl Acad Sci USA 106:14948. PubMed 3. Nguyen D, et al. 2011. Blood. 117:3793. PubMed
HepG2 lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody against SOCS3. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
The SOCS3 (suppressor of cytokine signaling-3) protein is a 25 kD cytoplasmic protein that contains a SOCS box and an SH2 domain. SOCS3 has been shown to be involved the negative regulation of cytokine signaling via JAK/STAT pathway. SOCS3 has been shown to inhibit IL-2, IL-3, IL-4, IL-6, erythropoietin, prolactin, and growth hormone. SOCS3 has also been shown to regulate erythropoiesis in the fetal liver. SOCS3 is induced induced by inflammation, erythropoietin, GM-CSF, IL-10, and IFN-γ. SOCS3 can be modified by JAK phophorylation which destabilizes the protein and ubiquitination which targets for destruction by the proteosome. SOCS3 has been shown to interact with the IGF-1 receptor, insulin receptor, gp130 receptor, erythropoietin receptor, leptin receptor, LIF receptor, JAK2, and elongin C. The SOC-25 monoclonal antibody recognizes human and mouse SOCS3 and has been shown to be useful for Western blotting.
Other Names:
Suppressor of cytokine signaling-3
Structure:
SSI or SOCS family, ESS, KIR, SH2 domains, SOCS box; 25 kD
Distribution:
Cytoplasm
Function:
Negative regulation of cytokines via JAK/STAT pathway by binding to tyrosine kinase receptors. Inhibition includes IL-2, IL-3, IL-4, IL-6, Epo, Prolactin, GH. Regulation of fetal liver erythropoiesis
Regulation:
Induced by inflammation, erythropoietin, GM-CSF, IL10, IFN-γ, destabilized when phosphorylated by JAK
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Purified anti-SOCS3
HepG2 lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody against SOCS3. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
