Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-SMC1L1 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
SMC1L1/SMC1 (structural maintenance of chromosome 1-like 1 (yeast)) is a 145-160 kD heterodimeric protein that is a member of the SMC family. This nuclear protein dissociates from chromosomes before mitosis but remains associated with kinetochore during mitosis. SMC1L1/SMC1 is located on the X-chromosome and is involved in mitotic chromosome dynamics. The SMC family includes SMC3, SMC1L1, and SMC1L2 which form part of the cohesion multiprotein complex. This protein can also form heterodimers consisting of SMC1L1/SMC3 or SMCL2/SMC3 and may also be involved in DNA repair. SMC1L1/SMC1 is phosphorylated by ATM after DNA damage; phosphorylation is also required for sister chromatid cohesion and correct segregation of chromosomes during cell division. SMC1L1/SMC1 associates with BRCA1, and SYCP2, and interacts directly with SMC3 in the cohesion complex (linked by RAD21 complexed to one STAG protein). The Poly6096 antibody recognizes the N-terminal region of human and mouse SMC1L1/SMC1 and has been shown to be useful for Western blotting and immunofluorescence staining.
Other Names:
Structural maintenance of chromosome 1-like 1 (yeast)
Structure:
SMC family, heterodimer; 145-160 kD
Distribution:
Nuclear, dissociates from chromosomes before mitosis. Remains associated with kinetochore during mitosis, located on X-chromosome
Function:
Mitotic chromosome dynamics. SMC family includes SMC3, SMC1L1, and SMC1L2 which form part of the cohesion multiprotein complex. Forms heterodimers of SMC1L1/SMC3 or SMCL2/SMC3, may also be involved in DNA repair
Regulation:
Phosphorylated by ATM after DNA damage. Distribution of SMC1L1 protein changes with position in the cell cycle
Modification:
Phosphorylation required for sister chromatid cohesion and correct segregation of chromosomes during cell division
Interaction:
BRCA1, SYCP2, interacts directly with SMC3 in the cohesion complex (linked by RAD21 complexed to one STAG protein)
Antigen References:
1. Zhou S, et al. 1998. P. Natl. Acad. Sci. USA 95:12906. 2. Yazdi P, et al. 2002. Genes Dev. 16:571. 3. Rocques P, et al. 1995. Hum. Mol. Genet. 4:243.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-SMC1L1/SMC1
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-SMC1L1 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
