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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Deford-Watts LM, et al. 2011. J. Immunol. 186:6839. PubMed.
MOLT4 total lysate (1% NP40) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-SLP76 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
SLP-76 is a human Src homology domain-containing leukocyte protein. This cytoplasmic adaptor phosphoprotein contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. The amino terminus of this protein contains three 17 amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P) as well as a proline rich region and is known to associate with many proteins involved in T and B cell signaling including Grb2, LAT, Vav1, SLAP-130, SHP-1, and phospholipase C gamma 2. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. Overexpression of SLP-76 has been shown to result in enhanced IL-2 transcription after TCR signaling. The H76 monoclonal antibody recognizes human SLP-76 and has been shown to be useful for Western blotting.
Other Names:
Lymphocyte cytoplasmic protein 2 (LCP2), SH2 domain containing leukocyte 76kD protein
Structure:
Phosphoprotein 76 kD containing SH2 domain in the C-terminus
Distribution:
Expressed in leukocytes (monocytes, T and B cells), thymus, spleen, placenta, and lung.
Function:
Adaptor molecule involved in B cell receptor and T cell receptor signaling
Modification:
Phosphorylation (Y113, Y128, Y145, Y423, Y426)
Interaction:
Grb2, LAT, Vav1, SLAP-130, SHP-1, phospholipase C gamma 2
Antigen References:
1. Jackman KJ, et al. 1995. J. Biol. Chem. 270:7029. 2. Wardenberg JB, et al. 1996. J. Biol. Chem. 271:19641. 3. Tuosto L, et al. 1996. J. Exp. Med. 184:1161. 4. Motto DG, et al. 1996. J. Exp. Med. 183:1937.
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Purified anti-SLP-76
MOLT4 total lysate (1% NP40) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-SLP76 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
