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Purified anti-SIK2 Antibody
Purified anti-SIK2 Antibody
636702 100 µg $195.00     
Product Details
Clone: S15G10
Isotype: Mouse IgG1, κ
Reactivity: Human
Immunogen: Recombinant human SIK2
Formulation: This antibody is prodived in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: This antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted at 4°C. 
Application:

WB-Quality tested
IP, IF - Reported in the literature
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated.
COA:
Enter Lot#:   
 Overnight nocodazole treated Hela cells
Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against SIK2 (clone S15G10), followed by Cy3 conjugated Goat anti-mouse IgG and DAPI.

Western blot analysis of extracts
Western blot analysis of extracts from Hela cells, using anti-SIK2, clone S15G10.


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Description:

SIK2 (Salt inducible kinase 2) belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and AMP-activated protein kinase (AMPK) subfamily, predicted molecular weight 104 kD. SIK2 is found in adipocytes and might be involved in metabolic regulation in adipose tissue. Sik2 phosphorylates ser794 of human insulin receptor substrate-1 (IRS1) in insulin-stimulated adipocytes, playing important roles in modulating the insulin-signaling cascade of adipocytes. Clone S15G10 has been shown to be useful for western blotting, immunoprecipitation, and immunoflorence staining of human SIK2 protein.
Other Names: Serine/threonine protein kinase SNF1 like kinase 2, Qin-induced kinase
Structure: Belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and AMP-activated protein kinase (AMPK) subfamily. SIK2 contains 1 protein kinase domain and 1 UBA domain.
Interaction: IRS 1, TORC2
Bioactivity/Activities: Involved in metabolic regulation in adipose tissue. Sik2 phosphorylates ser794 of human insulin receptor substrate-1(IRS1) in insulin-stimulated adipocytes, playing important roles in modulating the insulin-signaling cascade of adipocytes.
Antigen
References:

1. Horike N, et al. 2003. J. Biol. Chem. 278:18440.
GeneID: 23235
Latest Publications: View the latest SIK2 articles on HighwirePress.com
UniProt: View information about SIK2 on UniProt.org
Keywords: Purified anti-SIK2, S15G10, Purified, Serine/threonine protein kinase SNF1 like kinase 2, Qin-induced kinase , Human, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-SIK2
    Overnight nocodazole treated Hela cells

    Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against SIK2 (clone S15G10), followed by Cy3 conjugated Goat anti-mouse IgG and DAPI.

    Western blot analysis of extracts

    Western blot analysis of extracts from Hela cells, using anti-SIK2, clone S15G10.

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