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Purified anti-RCC1 Antibody
Purified anti-RCC1 Antibody
616401 50 µl (5 Western blots) ¥15,000     
616402 200 µl (20 Western blots) ¥45,000     
Product Details
Clone: Poly6164
Isotype: Rabbit IgG
Reactivity: Mouse, Human
Immunogen: Recombinant (partial), N-terminal
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20° C.
Application:

WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
 Hela cell nuclear extract was
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-RCC1 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.


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Description: RCC1 (regulator of chromosome condensation) is a 45 kD nuclear protein containing RCC1 repeats.This widely expressed protein associates with chromosomes and acts as a guanine nucleotide exchange factor (GEF) protein for Ras-related GTPase Ran. RCC1 is thought to maintain the Ran GTP gradient required for nucleocytoplasmic transport and spindle assembly across the interphase nuclear envelope and on condensed mitotic chromosomes. RCC1 regulates cell cycle and also plays a role in nuclear pore transport. Defects in RCC1 results in abnormal chromosome segregation and genomic instability. RCC1 is transcriptionally activated by Myc through E-box elements. RCC1 binds to chromatin in a binary complex with Ran. The Poly6164 antibody recognizes the N-terminal region of the human and mouse RCC1 protein and has been shown to be useful for Western blotting.
Other Names: Regulator of chromosome condensation
Structure: RCC1 repeats; 45 kD
Distribution: Nuclear, associates with chromosomes. Widely expressed
Function: Guanine nucleotide exchange factor (GEF) protein for Ras-related GTPase Ran. Maintains Ran GTP gradient required for nucleocytoplasmic transport and spindle assembly across the interphase nuclear envelope and on condensed mitotic chromosomes. Regulates ce
Regulation: Transcriptionally activated by Myc through E-box elements
Interaction: Binds to chromatin in a binary complex with Ran
Antigen
References: 		1. Ohtsubo M, et al. 1987. Genes Dev. 1:585.
2. Li H, et al. 2003. J. Cell Biol. 160:635.
3. Tsuneoka M, et al. 1997. Oncogene 14:2301.
GeneID: 1104
Latest Publications: View the latest RCC1 articles on HighwirePress.com
UniProt: View information about RCC1 on UniProt.org
Keywords: Purified anti-RCC1, Poly6164, Purified, Regulator of chromosome condensation , Mouse, Human, Immunology, Antibodies
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  • Purified anti-RCC1
    Hela cell nuclear extract was

    Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-RCC1 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

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