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This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol. Final antibody concentration 0.1 mg/ml.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000-1:10,000 dilutions. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
This antibody has been reported to be useful for Western blotting, immunoprecipitation, and immunofluorescence microscopy.
MOLT 4 extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Rb antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Rb (retinoblastoma 1) is a 105 kD nuclear protein containing RB-associated protein A and B domains. Rb acts as a transcriptional negative regulator of G1-phase cell cycle progression, and sequesters E2F and other transcription factors. Rb is unphosphorylated and inactive in G0, and becomes active upon hypophosphorylation by cyclinD-cdk4/6 in early G1. Rb is inactive with hyperphosphorylated by cyclinE-cdk2 late G1. Hypophosphorylated Rb form complexes with E2F, HDAC, cyclinD-cdk4/6, cyclinE-cdk2, PU 1, ATF 2, UBF, Elf 1, and c-Abl. The Poly6146 antibody recognizes the N-terminal region of human and mouse Rb and has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence staining.
Other Names:
Retinoblastoma 1
Structure:
RB-associated protein A, B domains; 105 kD
Distribution:
Nuclear
Function:
Transcriptional negative regulator of G1-phase cell cycle progression, sequesters E2F and other transcription factors
Regulation:
Unphosphorylated, inactive in G0, active form hypophosphorylated by cyclinD-cdk4/6 early G1. Inactive hyperphosphorylated by cyclinE-cdk2 late G1
Modification:
Phosphorylation
Interaction:
Only active hypophosphorylated form complexes with E2F, HDAC, cyclinD-cdk4/6, cyclinE-cdk2, PU 1, ATF 2, UBF, Elf 1, c-Abl
Antigen References:
1. Knudson AG, Jr. 1986. Annu. Rev. Genet. 20:231. 2. Lee E, et al. 1992. Nature 359:288. 3. Ezhevsky S, et al. 2001. Mol. Cell Biol. 21:4773. 4. Narita M, et al. 2003. Cell 113:703.
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Purified anti-Rb
MOLT 4 extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Rb antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
