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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical stainning of acetone-fixed frozen sections, in vitro stimulation and in vivo depletion.
Application References:
1. Kuhnlein P, et al. 1994. J. Immunol. 153:979. 2. Pelegri C, et al. 1996. Arthritis Rheum. 39(2):204. 3. Tsuchiya T, et al. 2002. Amer. J. of Respir. and Crit. Care Med. 165:1640.
LOU rat splenocytes stained withCD3 (1F4) APC and V65 purified, followed by anti-mouse IgG1 FITC
TCR γδ expression is observed on a subset of cells in the thymus, intestinal epithelium, skin, liver, peripheral lymphoid tissues, and peritoneum. The TCR γδ is involved in the antigen recognition of some bacterial or tumor-associated antigens presented by MHC class I. Immobilized V65 antibody has been reported to activate TCR-γδ-bearing T cells in vitro, and to deplete peripheral TCR-γδ-bearing T cells in vivo
.
Other Names:
TCR-γ/δ, γ/δ TCR
Structure:
Ig superfamily, associates with CD3 complex
Distribution:
T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum
Function:
Antigen recognition
Ligand Receptor:
Some bacterial or tumor antigens bound MHC class I
Antigen References:
1. Allison TJ, et al. 2001. Nature 411:820. 2. Chien YH, et al. 1996. Ann. Rev. Immunol.14:511. 3. Hiromatsu K, et al. 1992. J. Exp. Med. 175:49. 4. Bluestone JA, et al. 1991. Immunol. Rev. 120:5.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-rat TCR γ/δ
LOU rat splenocytes stained with CD3 (1F4) APC and V65 PE
Purified anti-rat TCR γ/δ
LOU rat splenocytes stained withCD3 (1F4) APC and V65 purified, followed by anti-mouse IgG1 FITC
