Purified anti-rat CD80 Antibody
Purified anti-rat CD80 Antibody
200202 500 µg $220.00 Qty:

Product Details

Clone: 3H5 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Purified Mouse IgG1, κ Isotype Ctrl
Reactivity: Rat
Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IP, IHC - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

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Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of ligand binding1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200208).


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Purified anti-rat CD80 Antibody 3H5 Image 1
LPS-stimulated LOU rat splenocytes (3 days) were stained with CD80 (clone 3H5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).

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CD80 is a 60 kD member of the immunoglobulin superfamily also known as B7-1, B7, and BB1. It is expressed on antigen presenting cells and activated T and B cells, and is a ligand for CD28 and CD152 (CTLA-4). CD80/CD28 interactions are important for T and B cell co-stimulation, while CD80/CD152 lead to the inhibition of T cell responses. The 3H5 antibody can block the co-stimulatory activity of CD80.

Other Names: B7-1, B7, BB1, Ly-53
Structure: Ig superfamily, 60 kD
Distribution: Antigen presenting cells, activated B cells and T cells
Function: T and B cell costimulation
Ligand Receptor: CD28, CD152 (CTLA-4)

1. Barclay, A.N. et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Linsley, P.S., et al. 1991. J. Exp. Med. 174:561.
3. Salomon, B., et al. 2001. Ann. Rev. Immunol. 19:225.

GeneID: 25408
Latest Publications: View the latest CD80 articles on
UniProt: View information about CD80 on
Keywords: Purified anti-rat CD80, 3H5, Purified, B7-1, B7, BB1, Ly-53, Rat, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunology, Antibodies

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Related Categories

Immunology > Rat Immunology > CD and Related Molecules > CD80 (B7-1) > 3H5
Immunology > Rat Immunology > B Cells > CD80 (B7-1) > 3H5
Immunology > Rat Immunology > Costimulatory Molecules > CD80 (B7-1) > 3H5
Immunology > Rat Immunology > T Regulatory Cells (TReg) > CD80 (B7-1)

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-rat CD80
    LPS-stimulated LOU rat splenocytes (3

    LPS-stimulated LOU rat splenocytes (3 days) were stained with CD80 (clone 3H5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).

  • Purified anti-rat CD80
    LPS-stimulated LOU rat splenocytes (3

    LPS-stimulated LOU rat splenocytes (3 days) were stained with CD80 (clone 3H5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).

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