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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of ligand binding1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200208).
LPS-stimulated LOU rat splenocytes (3 days) were stained with CD80 (clone 3H5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
CD80 is a 60 kD member of the immunoglobulin superfamily also known as B7-1, B7, and BB1. It is expressed on antigen presenting cells and activated T and B cells, and is a ligand for CD28 and CD152 (CTLA-4). CD80/CD28 interactions are important for T and B cell co-stimulation, while CD80/CD152 lead to the inhibition of T cell responses. The 3H5 antibody can block the co-stimulatory activity of CD80.
Other Names:
B7-1, B7, BB1, Ly-53
Structure:
Ig superfamily, 60 kD
Distribution:
Antigen presenting cells, activated B and T cells
Function:
T and B cell costimulation
Ligand Receptor:
CD28, CD152 (CTLA-4)
Antigen References:
1. Barclay, A.N. et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Linsley, P.S., et al. 1991. J. Exp. Med. 174:561. 3. Salomon, B., et al. 2001. Ann. Rev. Immunol. 19:225.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-rat CD80
LPS-stimulated LOU rat splenocytes (3 days) were stained with CD80 (clone 3H5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Purified anti-rat CD80
LPS-stimulated LOU rat splenocytes (3 days) were stained with CD80 (clone 3H5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
