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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemistry1,5 of acetone-fixed frozen sections and STUF-treated paraffin-embedded sections, immunoprecipitation2, in vitro and in vivo blocking of PMA-induced T cell blast aggregation and adhesion to HEV-derived endothelial cells and purified ICAM-1 protein2,3,4.
Application References:
1. Clark WM, et al. 1995. Mol. Chem. Neuropathol. 26:213. (IHC) 2. Tamatani T, et al. 1990. Int. Immunol. 2:165. (IP) 3. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. 4. Willenborg DO, et al. 1996. J. Immunol. 157:1973. 5. McKechnie NM, et al. 2002. IVOS 43:411. (IHC)
LOU rat splenocytes stained with purified 1A29, followed by anti-mouse IgG FITC
CD54 is an 85 kD protein also known as ICAM-1. It is a member of the immunoglobulin superfamily that is expressed on high endothelial venules in lymphoid tissue, peripheral blood monocytes, dendritic cells, granulocytes and thymic stromal cells. CD54 is weakly expressed on T and B cells, although expression can be increased by activation (especially on endothelial cells). ICAM-1 is an important protein in cell adhesion, inflammation and immune responses. CD54 has been shown to bind to CD11a/CD18 (LFA-1), CD11b/CD18, CD11c/CD18, CD43, fibrinogen, and hyaluronan. The 1A29 antibody has been reported to block T cell aggregation and binding of lymphocytes to HEV-derived endothelial cells and purified ICAM-1 protein.
Other Names:
ICAM-1
Structure:
Ig superfamily, 85 kD
Distribution:
High endothelial venules in lymphoid tissues, peripheral blood monocytes, dendritic cells, granulocytes, thymic stromal cells and weakly on T and B cells. Upregulated on activated cells and endothelial cells.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-rat CD54
LOU rat splenocytes stained with purified 1A29, followed by anti-mouse IgG FITC
Biotin anti-rat CD54
LOU rat splenocytes stained with biotinylated 1A29, followed by SAV-PE
