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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Dallman MJ, et al. 1984. Eur. J. Immunol. 14:260. 2. Vemeer LA, et al. 1994. Eur. J. Immunol. 24:585.
LOU rat splenocytes stained with OX-19 purified, followed by anti-mouse IgG PE
CD5 is a 67 kD glycoprotein, also known as Lyt-1, Ly-1 or Ly-12. It is a member of the scavenger receptor superfamily and primarily expressed on thymocytes and mature peripheral T lymphocytes, but not on NK cells, intestinal intraepithelial gamma/delta T cells. A CD5+ B cell subset (B-1) has not been characterized in that rat.
Other Names:
Ly-1, Tp67, Leu-1, T1, Lyt-1
Structure:
CD5 is a 67 kD glycoprotein of scavenger receptor superfamily. It is expressed on thymocytes, T cells and small subset of B cells.
Distribution:
Expressed on thymocytes, peripheral T cells, and some thymic dendritic cells. It should be noted that a CD5+ subset of B cells has not been characterized in the rat by OX-19. Most gamma/delta T cells do not express CD5.
Function:
Important in T-B cell interaction.
Ligand Receptor:
CD72
Antigen References:
1. Vermeer LA, et al. 1994. Eur. J. Immunol. 24:585.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-rat CD5
LOU rat splenocytes stained with OX-19 purified, followed by anti-mouse IgG PE
Biotin anti-rat CD5
LOU rat splenocytes stained with OX-19 biotin, followed by SAV-PE
