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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1.
Application References:
1. Smith T, et al. 2000. Nature Med. 6:62. (IHC)
LOU rat splenocytes stained with purified OX-33, followed by anti-mouse IgG FITC
CD45 is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. CD45RA is one of the CD45 isoforms with the molecular weight of 200-220 kD. It is expressed almost exclusively on B cells. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4.
Other Names:
Leukocyte common antigen, high molecular weight form
Structure:
Protein tyrosine phosphatase, type I transmembrane protein, hign molecular weight isoform approximately 200-220 kD
Distribution:
Primarily on B lymphocytes
Function:
B cell receptor signal transduction
Ligand Receptor:
Galectin-1, CD2, CD3, CD4
Antigen References:
1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155. 2. Woolett GR, et al. 1985. Eur. J. Immunol. 15:168.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-rat CD45RA
LOU rat splenocytes stained with purified OX-33, followed by anti-mouse IgG FITC
Biotin anti-rat CD45RA
LOU rat splenocytes stained with biotinylated OX-33, followed by Sav-PE
FITC anti-rat CD45RA
LOU rat splenocytes stained with OX-33 FITC
PE anti-rat CD45RA
LOU rat splenocytes stained with OX-33 PE
Alexa Fluor® 488 anti-rat CD45RA
LOU rat splenocytes stained with OX-33 Alexa Fluor® 488
