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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 to 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Krensky AM, et al. 1997 Methods Enzymol 287:162
Western blot analysis of recombinant Human RANTES/CCL5 protein (Catalog Number 580202) using Purified Anti-human RANTES Antibody, clone VL1.
RANTES (CCL5) is a member of the CC chemokine family. Its mature form consists of 68 amino acid residues. RANTES is a strong chemotractant for monocytes, NK cells, T cells and eosinphils. Rantes may have important role in mediating immune and inflammatory responses.
Other Names:
CCL5, C-C motif chemokine 5, Small-inducible cytokine A5, T-cell-specific protein RANTES, SIS-delta, T cell-specific protein P228, TCP228, Eosinophil-chemotactic cytokine, EoCP
Structure:
Human RANTES cDNA is a member of the C-C chemokine family with a MW of around 8kd. Human and mouse RANTES have 85% homology.
Distribution:
Reported to be produced by T cells, platelets, synovial fibroblasts and some tumor cells.
Function:
Able to chemoattract monocytes, NK cells, esosinphils and T cells.
Interaction:
Monocytes, NK, T and eosinphils.
Antigen References:
1. Von Luettichau, et al. 1996. Cytokine 8:89 2. Schall TJ, et al. 1991. Cytokine 3:165
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Purified anti-RANTES (CCL5)
Western blot analysis of recombinant Human RANTES/CCL5 protein (Catalog Number 580202) using Purified Anti-human RANTES Antibody, clone VL1.
Alexa Fluor® 647 anti-human RANTES (CCL5)
Human PBMCs cultured overnight with monensin, then surface stained with CD3 PE and intracellularly stained with VL1 Alexa Fluor® 647 (top panel), lower panel data shows the staining was specifically blocked by pre-incubation with purified VL1 antibody (gated on lymphocyte population)
