Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Formalin-fixed paraffin-embedded human breast tissue was stained with Poly6145 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Jurkat cell extract was western blotted with rabbit anti-Rad 17 (Ser645) antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Lane 1, control cells. Lane 2, Jurkat exposed to 500J UVB harvested at 1 hr.
Description:
Rad17 (also known as Rad17 homolog and Rad17-like protein) has four reported isoforms FM1-FM4, with molecular weights of approximately 71 kD, 73 kD, 57 kD, and 62 kD, respectively. This nuclear protein functions as a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. Rad17 is a component of the checkpoint clamp loader complex (Rad17/Rfc2-5) and recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin. Rad17 can be modified by phosphorylation and has been shown to associate with chromatin, DNA replication factor C, ATR, and Hus1. The Poly6145 recognizes human, mouse, and rat phosphorylated Rad17 (Ser645) and has been shown to be useful for Western blotting.
Other Names:
Rad17 homolog, Rad17-like protein
Structure:
Rad17 homolog, Rad17-like protein, four isoforms FM1-FM4, approximately 71 kD, 73 kD, 57 kD, and 62 kD, respectively
Distribution:
Nuclear
Function:
Cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. Component of checkpoint clamp loader complex (Rad17/Rfc2-5), recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin
Modification:
Phosphorylation
Interaction:
Chromatin, DNA replication factor C, ATR, Hus1
Antigen References:
1. Parker, A., et al., 1998. J. Biol. Chem. 273:18340. 2. Chen, M., et al., 2001. Gene. 277:145. 3. Bao, S., et al., 2001. Nature 411:969. 4. Wang, X., et al., 2003. Genes Dev. 17:965.
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