This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-PYK2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
PYK2, a 116 kD proline-rich non-receptor tyrosine kinase also known as FAK2 or PTK2B, is ubiquitously expressed in the cytoplasm. PYK2 is activated in response to integrin ligation, CD3 or TCR ligation, growth factors, cytokines, chemokines, and cellular stress. This kinase has been shown to regulate ion channels, vesicular transport, participate in T- and B-cell receptor signaling, and play a role in apoptosis. PYK2 is activated through phosphorylation following increased intracellular calcium and can be dephosphorylated by calcitonin, and protein tyrosine phosphatase (non-receptor) type 12 to decrease kinase activity. PYK2 has been shown to interact with Grb2, SHP1, integrin β3, Fyn, c-Src, talin, paxillin, Vav-1, Lyn, and HIC5. The Poly6053 antibody recognizes the N-terminal region of human, mouse, and rat PYK2 and has been shown to be useful for Western blotting.
Tyrosine family of protein kinases, FAK subfamily; 116 kD
Distribution:
Cytoplasm, ubiquitously expressed
Function:
Non-receptor proline-rich tyrosine kinase. Activated in response to ligation of integrins, CD3, TCR, CD28, growth factors, cytokines, chemokines, stress. Regulates ion channels, vesicular transport, participates in T- and B-cell receptor signaling, apopto
Regulation:
Activated through phosphorylation via increases intracellular calcium concentrations. Dephosphorylated by calcitonin, protein tyrosine phosphatase (non-receptor) type 12
