Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Lang, D., et al. 2011. AM J. Physiol Heart Circ Physiol. 301:975. PubMed.
Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-PYK2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
PYK2, a 116 kD proline-rich non-receptor tyrosine kinase also known as FAK2 or PTK2B, is ubiquitously expressed in the cytoplasm. PYK2 is activated in response to integrin ligation, CD3 or TCR ligation, growth factors, cytokines, chemokines, and cellular stress. This kinase has been shown to regulate ion channels, vesicular transport, participate in T- and B-cell receptor signaling, and play a role in apoptosis. PYK2 is activated through phosphorylation following increased intracellular calcium and can be dephosphorylated by calcitonin, and protein tyrosine phosphatase (non-receptor) type 12 to decrease kinase activity. PYK2 has been shown to interact with Grb2, SHP1, integrin β3, Fyn, c-Src, talin, paxillin, Vav-1, Lyn, and HIC5. The Poly6053 antibody recognizes the N-terminal region of human, mouse, and rat PYK2 and has been shown to be useful for Western blotting.
Tyrosine family of protein kinases, FAK subfamily; 116 kD
Distribution:
Cytoplasm, ubiquitously expressed
Function:
Non-receptor proline-rich tyrosine kinase. Activated in response to ligation of integrins, CD3, TCR, CD28, growth factors, cytokines, chemokines, stress. Regulates ion channels, vesicular transport, participates in T- and B-cell receptor signaling, apopto
Regulation:
Activated through phosphorylation via increases intracellular calcium concentrations. Dephosphorylated by calcitonin, protein tyrosine phosphatase (non-receptor) type 12
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-PYK2
Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-PYK2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
