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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
HepG2 cell nuclear extract (lane 1) and whole cell extract from mouse splenocytes (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-PXR antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
PXR (also known as orphan nuclear receptor PXR, pregnane X receptor, orphan nuclear receptor PAR1, and steroid and xenobiotic receptor (SXR)) is a member of the nuclear hormone receptor family, NR1 subfamily containing a zinc finger domain. Alternatively spliced variants of this receptor have been reported. This nuclear protein has a molecular weight of 50 kD and is highly expressed in liver, colon, and small intestine. PXR is an orphan receptor whose natural ligand is probably pregnane. PXR binds to the promoter response element in cytochrome p450 gene monooxygenase CYP3A4 and activates expression in response to variety of endobiotics and xenobiotics. PXR is activated by naturally occurring steroids such as pregnenolone and progesterone and can also be activated by dexamethasone and rifampicin. PXR forms a heterodimer with RXR. The Poly6169 antibody recognizes human and mouse PXR and has been shown to be useful for Western blotting.
Other Names:
Orphan nuclear receptor PXR, Pregnane X receptor, Orphan nuclear receptor PAR1, Steroid and xenobiotic receptor (SXR)
Nuclear. Highly expressed in liver, colon, small intestine
Function:
Orphan receptor, natural ligand probably pregnan. Binds to promoter response element in cytochrome p450 gene monooxygenase CYP3A4. Activates expression in response to variety of endobiotics, xenobiotics
Regulation:
Activated by naturally occurring steroids such as pregnenolone and progesterone, also activated by dexamethasone and rifampicin
Interaction:
Heterodimer with RXR
Antigen References:
1. Lehmann J, et al. 1998. J. Clin. Invest. 102:1016. 2. Bertilsson G, et al. 1998. P. Natl. Acad. Sci. USA 95:12208. 3. Watkins R, et al. 2001. Science 292:2329.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-PXR
HepG2 cell nuclear extract (lane 1) and whole cell extract from mouse splenocytes (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-PXR antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
