Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5ml antibody dilution buffer for each mini-gel. For IHC, use a 15 µg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Formalin-fixed paraffin-embedded human kidney tissue was stained with 3H7 at 15 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Extracts from human PBMC were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody against PTBP1 (clone 3H7). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Description:
The 3H7 monoclonal antibody recognizes human PTBP1 also known as Polypyrimidine tract binding protein 1, Heterogeneous nuclear ribonucleoprotein I, and 57 kDa RNA-binding protein. PTBP1 is a ubiquitously expressed nuclear protein with a predicted molecular weight approximately 59.6 kD. The polypyrimidine tract binding protein (PTB) is an important regulator of alternative splicing that also affects mRNA localization, stabilization, polyadenylation, and translation. PTBP1 has been shown to interact with FUS, Caspase3, PKCa, Raver1, and HnRNP-L protein. The PTB gene contains 15 exons and spans 13.5 kb. The 3H7 antibody has been shown to be useful for Western blotting.
Other Names:
Polypyrimidine tract binding protein 1, Heterogeneous nuclear ribonucleoprotein I, hnRNP I, 57 kDa RNA-binding protein PPTB-1, PTB
Structure:
PTBP1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). PTBP1 contains four RNA recognition motifs (RRMs). Nuclear protein, predicted molecular weight approximately 59.6 kD
Distribution:
Nucleus, ubiquitously expressed
Function:
Plays a role in pre-mRNA splicing. Binds to the polypyrimidine tract of introns. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
Interaction:
FUS, Caspase3, PKCa, Raver1, and HnRNP-L
Antigen References:
1. Gil,A., et al. 1991. Genes Dev. 5 (7): 1224. 2. Ghetti, A., et al. 1992. Acids Res. 20:3671. 3. Romanelli, M. G., et al. 2000. Gene 255:267.
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