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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Witkowski A. 2007. J. Biol. Chem. doi:10.1074/jbc.M701486200.PubMed 2. Da Silva-Azevedo L, et al. 2008. J Physiol.PubMed 3. Patel N, et al. 2010. Proc Natl Acad Sci USA> 107:2503. PubMed
MCF7 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-prohibitin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Prohibitin is a 30 kD member of the band 7 family containing a SPFH domain. Prohibitin is localized the nucleus and mitochondrial inner cell membrane. This protein functions as a negative regulator of cell proliferation associated with senescence and cell death and a transcriptional repressor via recruitment of Brg-1, Brm or N-CoR, or HDAC1. Prohibitin can also function as a membrane-bound chaperone for the assembly of the mitochondrial respiratory chain complex. Prohibitin is induced by metabolic stress caused by imbalance in synthesis of mitochondrial-, nuclear-encoded mitochondrial proteins. Prohibitin can be modified by phosphorylation and ubiquitination. This protein has been shown to interact with Rb, Brg-1, Brm, N-CoR, HDAC1, and PHB2. The Poly6031 antibody recognizes the C-terminal region of human prohibitin and has been shown to be useful for Western blotting.
Other Names:
PHB, PHB1
Structure:
Band 7 family, SPFH domain; 30 kD
Distribution:
Nuclear, mitochondrial inner cell membrane
Function:
Negative regulator of cell proliferation associated with senescence and cell death. Repression of transcription via recruitment of Brg-1, Brm or N-CoR, HDAC1. Membrane-bound chaperone for assembly of mitochondrial respiratory chain complex
Regulation:
Induced by metabolic stress caused by imbalance in synthesis of mitochondrial-, nuclear-encoded mitochondrial proteins
Modification:
Phosphorylation, Ubiquitination
Interaction:
RB, Brg-1, Brm, N-CoR, HDAC1, PHB2
Antigen References:
1. White J, et al. 1991. Genomics 11:228. 2. Nijtmans L, et al. 2002. Cell Mol. Life Sci. 59:143. 3. Wang S, et al. 2002. Oncogene 21:8388. 4. Thompson W, et al. 2003. Biol. Reprod. 69:254.
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Purified anti-Prohibitin
MCF7 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-prohibitin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
