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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended to use sensitive techniques such as immunoprecipitation-Western blot analysis.
COA:
PLK-1 was immunoprecipitated from Hela cell extract using monoclonal anti-PLK-1, clone 3F8. Immunoprecipitates were resolved by electrophoresis, transferred to nitrocellulose, and probed with the polyclonal anti-PLK-1, Poly6185.
PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The Poly6185 antibody recognizes human PLK-1 and has been shown to be useful for Western blotting and immunoprecipitation.
Other Names:
Serine/Threonine protein kinase PLK, Polo-like kinase (PLK), Serine-threonine protein kinase 13
Structure:
Serine/Threonine family of protein kinases, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila). Contains two polo box domains. Predicted molecular weight 68 kD
Distribution:
Nuclear protein, highly expressed in placenta and colon
Function:
Regulates cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. May be required for cell division. Depletion of PLK-1 results in apoptosis
Regulation:
Upregulated by growth stimulating agents. Regulated by cell cycle position (highest in G2/M phase and declines to nearly undetectable levels after mitosis and throughout G1)
Modification:
Phosphorylation
Interaction:
Interacts with nuclear distribution gene C
Antigen References:
1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249. 2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793. 3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.
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Purified anti-PLK-1
PLK-1 was immunoprecipitated from Hela cell extract using monoclonal anti-PLK-1, clone 3F8. Immunoprecipitates were resolved by electrophoresis, transferred to nitrocellulose, and probed with the polyclonal anti-PLK-1, Poly6185.
