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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µl antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The optimal concentration should be determined by titration for each individual assay of interest.
Jurkat total cell lysates were resloved by electrophoresis, transferred to nitrocellulose and probed either with anti-phosphor-PKA (T638) antibody solely (Lane 1), anti-phosphor-PKA (T638) antibody plus phosphor-peptide (Lane 2), or with the anti-phosphor-PKA (T638) antibody plus non-phosphor-peptide (Lane 3). Proteins were visualized using an HRP donkey anti-rabbit secondary antibody and a chemiluminescence detection system.
Protein kinase C alpha (PKCα), also known as PRKCA, refers to both a human gene and the protein that is encoded by it. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca2+ handling in myocytes. The purified anti-PKCα Phospho (Thr638) antibody detects PKCα only when phosphorylated at threonine 638.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. The protein encoded by this gene is one of the PKC family members. The most significant structu
Distribution:
Universal expression in both cytosol and nucleus. PKCα has been widely studied in the tissues of many organisms including Drosophila, Xenopus, cow, dog, chicken, human, monkey, mouse, pig, and rabbit.
Function:
PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca2+ handling in myocytes.
Cellular Targets:
PKC family members phosphorylate a wide variety of protein targets.
Antigen References:
1. Seo HR, et al. 2004. Exp. Mol. Med. 36:292. 2. Wientzek M, et al. 1997. Mol. Cell. Biochem. 166:11. 3. Baier G and Wagner J. 2009. Curr. Opin. Cell Biol. 21:262. 4. Reyland NE. 2009. Front. Biosci. 14:2386.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-PKCα Phospho (Thr638)
Jurkat total cell lysates were resloved by electrophoresis, transferred to nitrocellulose and probed either with anti-phosphor-PKA (T638) antibody solely (Lane 1), anti-phosphor-PKA (T638) antibody plus phosphor-peptide (Lane 2), or with the anti-phosphor-PKA (T638) antibody plus non-phosphor-peptide (Lane 3). Proteins were visualized using an HRP donkey anti-rabbit secondary antibody and a chemiluminescence detection system.
