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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 25 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-PKC monoclonal antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
PKC, also known as protein kinase C, belongs to a kinase family involved in intracellular signaling processes. PKC isoforms are divided into three groups known as conventional (including PKCα, PKCβ1, PKCβ2 and PKCγ), novel (including PKCε, μ, θ, δ, η), and atypical PKC isoforms (such as ξ). Conventional PKC isoforms require calcium and diacylglycerol for activation, novel isoforms require diacylglycerol, and atypical isoforms require neither calcium nor diacylglycerol. Once activated by phosphorylation, the PKCs phosphorylate a large variety of substrates involved in cell differentiation, proliferation, and activation. The PKC0103 antibody recognizes human, mouse, and rat PKC and is useful for Western blotting and immunoprecipitation.
Other Names:
Protein kinase C
Structure:
Serine/threonine kinase containing a regulatory and catalytic domain composed of a number of conserved regions interspersed with variable regions. At least 12 isoforms exist with differences in structure, substrates, expression and localization.
Distribution:
High expression in brain, isoforms vary in tissue expression
Function:
PKC isoforms are involved in a variety of intracellular signaling processes mediated through phospholipids hydrolysis. Once activated by phosphorylation, the PKCs phosphorylate a large variety of substrates involved in cell differentiation, proliferation,
Modification:
Phosphorylation
Antigen References:
1. Liu WS, et al. 1998. Cell Signal 10:529. 2. Way KJ, et al. 2000. Trends Pharmacol. Sci. 21:181.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-PKC
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-PKC monoclonal antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
