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Purified anti-PERK Phospho (Ser713) Antibody
Purified anti-PERK Phospho (Ser713) Antibody
649401 100 µl (5 Western blots) ¥25,000     
649402 400 µl (20 Western blots) ¥70,000     
Product Details
Clone: Poly6494
Isotype: Rabbit polyclonal
Reactivity: Human
Immunogen: Modified synthetic peptide
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store at -20°C.
Application:

WB - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 20 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
Notes:

The optimal concentration should be determined by titration for each individual assay of interest.
Untransfected 293T cells (lane 2)
Untransfected 293T cells (lane 2) or transfected 293T cells (lane 4) were treated with 1 µM of Thapsigargin for 30 min to induce ER stress. Untreated 293T (lane 1) and 293 transfectant (lane 3) was used as the control. Lystates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PERK phosphylated (Ser713) antibody. Protein was visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminesce detection system.


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Description:

PERK (protein kinase-like endoplasmic reticulum kinase) is a type I membrane protein located in the endoplasmic reticulum (ER). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress. PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain. Phosphorylation of PERK at Ser713 can be used as a marker for its activation status.
Other Names: eukaryotic translation initiation factor 2 alpha kinase 3; PRKR-like endoplasmic reticulum kinase; heme-regulated EIF2-alpha kinase
Structure: 1115 amino acids; 125 kD predicted. The observed MW for phosphor-PERK is approximately 150-170 kD.
Distribution: Endoplasmic reticulum membrane; Single-pass type I membrane protein
Function: Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
Interaction: Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells.
Antigen
References:

1. Shi Y, et al. 1998. Mol. Cell. Biol. 18:7499.
2. Shi Y, et al. 1999. J. Biol. Chem. 274:5723.
3. Cybulsky AY, et al. 2005. J. Biol. Chem. 280:24396.
4. Kohno K. 2010. J. Biochem. 147:27.
GeneID: 9451
Latest Publications: View the latest PERK Phospho articles on HighwirePress.com
UniProt: View information about PERK Phospho on UniProt.org
Keywords: Purified anti-PERK Phospho (Ser713), Poly6494, Purified, eukaryotic translation initiation factor 2 alpha kinase 3; PRKR-like endoplasmic reticulum kinase; heme-regulated EIF2-alpha kinase, Human, Immunology, Antibodies
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  • Purified anti-PERK Phospho (Ser713)
    Untransfected 293T cells (lane 2)

    Untransfected 293T cells (lane 2) or transfected 293T cells (lane 4) were treated with 1 µM of Thapsigargin for 30 min to induce ER stress. Untreated 293T (lane 1) and 293 transfectant (lane 3) was used as the control. Lystates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PERK phosphylated (Ser713) antibody. Protein was visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminesce detection system.

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