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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, immunofluorescence microscopy9, and Western blotting10.
Application References:
1. Ogata K, et al. 1985. J. Immunol. 135:2623. 2. Garcia R, et al. 1989. Am. J. Pathol. 134:733. (IHC) 3. Landberg G, et al. 1990. Exp. Cell. Res. 187:111. (ICFC) 4. Waseem N, et al. 1990. J. Cell Sci. 96:121. 5. Yu C, et al. 1991. Histopathology 19:29. (IHC) 6. Wilkins B, et al. 1992. J. Pathol. 166:45. (IHC) 7. Yang W, et al. 1996. Human Pathol. 27:70. 8. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329. 9. Chou HYE, et al. 2006. J. Biol. Chem. 10:1074. (IF) 10. Fulvio M D, et al. 2006. Oncogene 25:3932. (WB) 11. Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937. PubMed 12. Chou HY,et al.2006.J. Biol. Chem.281:15201. PubMed 13. Di Fulvio M,et al.2006. Oncogene.25:3032. PubMed
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
PCNA, also known as DNA polymerase δ auxiliary protein, is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase δ processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody. The PC10 monoclonal antibody recognizes human and mouse PCNA (cross-reactive with other species) and has been shown to be useful for flow cytometry, immunohistochemistry (frozen and formalin-fixed, paraffin-embedded tissues), immunoprecipitation, Western blotting, and immunofluorescence staining.
Other Names:
Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein
RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
Modification:
Ubiquitination, Sumoylation
Interaction:
PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
Antigen References:
1. Travali S, et al. 1989. J. Biol. Chem. 264:7466. 2. Waseem N, et al. 1990. J. Cell Sci. 96:121. 3. Hall P, et al. 1990. J. Pathol. 162:285. 4. Landberg G, et al. 1991. Cancer Res. 51:4570. 5. Woods A, et al. 1991. Histopathol. 19:21. 6. Hoege C, et al. 2002. Nature 419:135. 7. Yue H, et al. 2003. World J. Gastroenterol. 9:377. 8. Shan B, et al. 2003. J. Biol. Chem. 278:44009.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-PCNA
MOLT-4 cells fixed in 70% ethanol then stained with PC10 PE
Purified anti-PCNA
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Alexa Fluor® 488 anti-PCNA
MOLT-4 cells fixed in 70% ethanol then stained with PC10 Alexa Fluor® 488 or MOPC-173 Alexa Fluor® 488
Alexa Fluor® 647 anti-PCNA
Human peripheral blood lymphocytes fixed with 70% ethanol, then intracellular stained with PC10 Alexa fluor® 647
