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Purified Anti-PCNA Antibody
Material Safety Data Sheet (MSDS) Purified Anti-PCNA Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-PCNA Antibody
307901 25 µg $50.00     
307902 100 µg $95.00     
Clone: PC10
Isotype: Mouse IgG2a, κ
Reactivity: Human, Mouse, Rat Cross-Reactivity*: Other species
Immunogen: Recombinant rat PCNA
Formulation: Phosphate-buffered solution, pH7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C.
Application: FC - Quality Tested
IHC (Frozen/ Paraffin)2,5,6*, IP*, WB10*, IF9 * - *This application has been reported in the literature.
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and/or Western blotting. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. For Western blotting, the suggested use of this reagent is ≤ 1.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, immunofluorescence microscopy9, and Western blotting10.
Application
References:
1. Ogata, K., et al., 1985. J. Immunol. 135:2623.
2. Garcia, R., et al., 1989. Am. J. Pathol. 134:733.
3. Landberg, G., et al., 1990. Exp. Cell. Res. 187:111.
4. Waseem, N., et al., 1990. J. Cell Sci. 96:121.
5. Yu, C., et al., 1991. Histopathology 19:29.
6. Wilkins, B., et al., 1992. J. Pathol. 166:45.
7. Yang, W., et al., 1996. Human Pathol. 27:70.
8. Galkowska, H., et al., 1996. Vet. Immunol. Immunopathol. 53:329.
9. Chou, H-Y. E., et al., 2006. J. Biol. Chem. 10:1074.
10. Fulvio, M. D., et al., 2006. Oncogene 25:3932.
11. Eswarakumar, V. P. and Schlessinger, J., 2007. Proc. Natl. Acad. Sci. USA 104:3937.PubMed
12. Chou, HY.,et al.2006.J Biol Chem.281:15201. PubMed
13. Di Fulvio, M.,et al.2006. Oncogene.25:3032.PubMed
Hela cell nuclear extract was

Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.


Description: PCNA, also known as DNA polymerase δ auxiliary protein, is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase δ processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody. The PC10 monoclonal antibody recognizes human and mouse PCNA (cross-reactive with other species) and has been shown to be useful for flow cytometry, immunohistochemistry (frozen and formalin-fixed, paraffin-embedded tissues), immunoprecipitation, Western blotting, and immunofluorescence staining.
Other Names: Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein
Structure: DNA-polymerase sliding clamp, trimeric ring; 36 kD
Distribution: Nuclear, all proliferating cells
Function: RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
Modification: Ubiquitination, Sumoylation
Interaction: PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
Antigen
References:
1. Travali, S., et al., 1989. J. Biol. Chem. 264:7466.
2. Waseem, N., et al., 1990. J. Cell Sci. 96:121.
3. Hall, P., et al., 1990. J. Pathol. 162:285.
4. Landberg, G., et al., 1991. Cancer Res. 51:4570.
5. Woods A., et al., 1991. Histopathol. 19:21.
6. Hoege, C., et al., 2002. Nature 419:135.
7. Yue, H., et al., 2003. World J. Gastroenterol. 9:377.
8. Shan, B., et al., 2003. J. Biol. Chem. 278:44009.
APC Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
Biotin Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC, ELISA, IHC, IF, WB
FITC Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
HRP Goat anti-mouse IgG (minimal x-reactivity)Poly4053ELISA,IHC,WB
Purified Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICC, IF, IHC, IP, WB
PE Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
Streptavidin-FITCStreptavidinFC,ICFC
Streptavidin-HRPStreptavidinELISA,ELISPOT,IHC,WB
Streptavidin-PEStreptavidinFC,ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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