This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB1
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Yao, H., et al., 2006. Oncogene 25:2285.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-phospho-paxillin (Tyr31). Lane 1, Hela cells serum-starved overnight; Lane 2, Hela cells serum-starved overnight following serum addition for 4 hrs. Lane 2 shows an upregulation of phosphorylated paxillin after serum addition. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
Paxillin (also known as peroxisomal membrane protein 3) is a cytoplasmic protein with LIM metal-binding repeat homology and LIM domains. There are three isoforms of this protein designated as α, β, γ; approximate molecular weight 68 kD. Paxillin is localized to actin-membrane attachment at sites of cell adhesion to extracellular matrix (focal adhesion). This protein undergoes cell adhesion-dependent tyrosine phosphorylation. Paxillin has been shown to interact with FAK, vinculin, src, Crk, p210BRC / ABL, talin, and integrin-β1. The Poly6008 antibody recognizes human phosphorylated paxillin (Tyr31) and has been shown to be useful for Western blotting.
1. Leventhal, P., et al., 1997. J. Biol. Chem. 272:5214. 2. Mazaki, Y., et al., 1997. J. Biol. Chem. 272:7437. 3. Kirchner, J., et al., 2003. J. Cell Sci. 116:975. 4. Tang, D., et al., 2003. J. Physiol. 42:858.
