Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Yao, H., et al., 2006. Oncogene 25:2285. (WB) PubMed 2. Li, D., et al., 2009. Tumori 95:769 (WB) PubMed
Extract from human PBMC activated with PHA and IL-2 for 5 days was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-paxillin (Tyr118) antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Lane 1, PHA and IL-2 activated PBMC. Lane 2, PHA and IL-2 activated PBMC crosslinked with a-CD3 antibody for 5 min showing an upregulation of phospho-paxillin. In addition to the specific paxillin band, this polyclonal antibody recognizes an unknown protein with an apparent mass of 100 kD.
Description:
Paxillin (also known as peroxisomal membrane protein 3) is a cytoplasmic protein with LIM metal-binding repeat homology and LIM domains. There are three isoforms of this protein designated as α, β, γ; approximate molecular weight 68 kD. Paxillin is localized to actin-membrane attachment at sites of cell adhesion to extracellular matrix (focal adhesion). This protein undergoes cell adhesion-dependent tyrosine phosphorylation. Paxillin has been shown to interact with FAK, vinculin, src, Crk, p210BRC / ABL, talin, and integrin-β1. The Poly6009 antibody recognizes human phosphorylated paxillin (Tyr118) and has been shown to be useful for Western blotting.
1. Leventhal, P., et al., 1997. J. Biol. Chem. 272:5214. 2. Mazaki, Y., et al., 1997. J. Biol. Chem. 272:7437. 3. Kirchner, J., et al., 2003. J. Cell Sci. 116:975. 4. Tang, D., et al., 2003. J. Physiol. 42:858.
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