Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for relevant formats of this clone) include: immunofluorescence microscopy1, immunoprecipitation2 and immunohistochemical staining2 of formalin-fixed paraffin embedded sections.
Application References:
1. Yao, H., et al., 2006. Oncogene 25:2285. (IF, WB) PubMed 2. Li, D., et al., 2009. Tumori 95:769 (IP, IHC) PubMed
Immunofluorescent microscope analysis of Hela cells using anti-Paxillin monoclonal antibody (clone PAX227), followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
Hela total lysate (1% NP40) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-paxillin antibody.
Description:
Paxillin (also known as peroxisomal membrane protein 3) is a cytoplasmic protein with LIM metal-binding repeat homology and LIM domains. There are three isoforms of this protein designated as α, β, γ; approximate molecular weight 68 kD. Paxillin is localized to actin-membrane attachment at sites of cell adhesion to extracellular matrix (focal adhesion). This protein undergoes cell adhesion-dependent tyrosine phosphorylation. Paxillin has been shown to interact with FAK, vinculin, src, Crk, p210BRC / ABL, talin, and integrin-β1. The PAX227 monoclonal antibody recognizes human and mouse paxillin and has been shown to be useful for Western blotting.
1. Leventhal, P., et al., 1997. J. Biol. Chem. 272:5214. 2. Mazaki, Y., et al., 1997. J. Biol. Chem. 272:7437. 3. Kirchner, J., et al., 2003. J. Cell Sci. 116:975. 4. Tang, D., et al., 2003. J. Physiol. 42:858.
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