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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for relevant formats of this clone) include: immunofluorescence microscopy1, immunoprecipitation2 and immunohistochemical staining2 of formalin-fixed paraffin embedded sections.
Application References:
1. Yao H, et al. 2006. Oncogene 25:2285. (IF, WB) PubMed 2. Li D, et al. 2009. Tumori 95:769 (IP, IHC) PubMed
10 ng of Paxillin recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Paxillin antibody (clone PAX227). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and chemiluminescence detection.
Immunofluorescent microscope analysis of Hela cells using anti-Paxillin monoclonal antibody (clone PAX227), followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
Paxillin (also known as peroxisomal membrane protein 3) is a cytoplasmic protein with LIM metal-binding repeat homology and LIM domains. There are three isoforms of this protein designated as α, β, γ; approximate molecular weight 68 kD. Paxillin is localized to actin-membrane attachment at sites of cell adhesion to extracellular matrix (focal adhesion). This protein undergoes cell adhesion-dependent tyrosine phosphorylation. Paxillin has been shown to interact with FAK, vinculin, src, Crk, p210BRC / ABL, talin, and integrin-β1. The PAX227 monoclonal antibody recognizes human and mouse paxillin and has been shown to be useful for Western blotting.
1. Leventhal P, et al. 1997. J. Biol. Chem. 272:5214. 2. Mazaki Y, et al. 1997. J. Biol. Chem. 272:7437. 3. Kirchner J, et al. 2003. J. Cell Sci. 116:975. 4. Tang D, et al. 2003. J. Physiol. 42:858.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Paxillin
10 ng of Paxillin recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Paxillin antibody (clone PAX227). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and chemiluminescence detection.
Immunofluorescent microscope analysis of Hela cells using anti-Paxillin monoclonal antibody (clone PAX227), followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
