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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a starting dilution of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
MOLT-4 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-paxillin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Immunofluorescent microscope analysis of Hela cells using anti-Paxillin polyclonal antibody (red). Nuclei were stain with DAPI (blue).
Paxillin (also known as peroxisomal membrane protein 3) is a cytoplasmic protein with LIM metal-binding repeat homology and LIM domains. There are three isoforms of this protein designated as α, β, γ; approximate molecular weight 68 kD. Paxillin is localized to actin-membrane attachment at sites of cell adhesion to extracellular matrix (focal adhesion). This protein undergoes cell adhesion-dependent tyrosine phosphorylation. Paxillin has been shown to interact with FAK, vinculin, src, Crk, p210BRC / ABL, talin, and integrin-β1. The Poly6007 antibody recognizes human, mouse, and rat paxillin and has shown to be useful for Western blotting.
1. Leventhal P, et al. 1997. J. Biol. Chem. 272:5214. 2. Mazaki Y, et al. 1997. J. Biol. Chem. 272:7437. 3. Kirchner J, et al. 2003. J. Cell Sci. 116:975. 4. Tang D, et al. 2003. J. Physiol. 42:858.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Paxillin
MOLT-4 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-paxillin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Immunofluorescent microscope analysis of Hela cells using anti-Paxillin polyclonal antibody (red). Nuclei were stain with DAPI (blue).
