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Purified Anti-Paxillin Antibody
Material Safety Data Sheet (MSDS) Purified Anti-Paxillin Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-Paxillin Antibody
600701 50 µl (5 Western blots) $75.00     
600702 200 µl (20 Western blots) $225.00     
Clone: Poly6007
Isotype: Rabbit IgG
Reactivity: Mouse, Rat, Human
Immunogen: Full length recombinant protein (human)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20° C.
Application:

WB1,2, IF1

Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a starting dilution of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.

Application
References:

1. Yao, H., et al., 2006. Oncogene 25:2285. (WB, IF) PubMed
2. D'Onofrio, M-F., et al., 2007. J. Biol. Chem. doi:10.1074/jbc.M701861200.
3. Proszynski, TJ., et al. 2009. Proc Natl Acad Sci U S A. 106:18373. PubMed

MOLT-4 cell extract was resolved

MOLT-4 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-paxillin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Immunofluorescent microscope analysis of Hela

Immunofluorescent microscope analysis of Hela cells using anti-Paxillin polyclonal antibody (red). Nuclei were stain with DAPI (blue).


Description:

Paxillin (also known as peroxisomal membrane protein 3) is a cytoplasmic protein with LIM metal-binding repeat homology and LIM domains. There are three isoforms of this protein designated as α, β, γ; approximate molecular weight 68 kD. Paxillin is localized to actin-membrane attachment at sites of cell adhesion to extracellular matrix (focal adhesion). This protein undergoes cell adhesion-dependent tyrosine phosphorylation. Paxillin has been shown to interact with FAK, vinculin, src, Crk, p210BRC / ABL, talin, and integrin-β1. The Poly6007 antibody recognizes human, mouse, and rat paxillin and has shown to be useful for Western blotting.

Other Names: Peroxisomal membrane protein 3
Structure: LIM metal-binding repeat homology, LIM domains, α, β, γ-isoforms; 68 kD
Distribution: Cytoplasm
Function: Actin-membrane attachment at sites of cell adhesion to extracellular matrix (focal adhesion)
Regulation: Cell adhesion-dependent tyrosine phosphorylation
Modification: Phosphorylation
Interaction: FAK, vinculin, src, Crk, p210BRC / ABL, talin, integrin-β1
Antigen
References:

1. Leventhal, P., et al., 1997. J. Biol. Chem. 272:5214.
2. Mazaki, Y., et al., 1997. J. Biol. Chem. 272:7437.
3. Kirchner, J., et al., 2003. J. Cell Sci. 116:975.
4. Tang, D., et al., 2003. J. Physiol. 42:858.

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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