This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB1,2, IF1
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a starting dilution of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Yao, H., et al., 2006. Oncogene 25:2285. (WB, IF) PubMed 2. D'Onofrio, M-F., et al., 2007. J. Biol. Chem. doi:10.1074/jbc.M701861200. 3. Proszynski, TJ., et al. 2009. Proc Natl Acad Sci U S A. 106:18373. PubMed
MOLT-4 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-paxillin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Immunofluorescent microscope analysis of Hela cells using anti-Paxillin polyclonal antibody (red). Nuclei were stain with DAPI (blue).
Description:
Paxillin (also known as peroxisomal membrane protein 3) is a cytoplasmic protein with LIM metal-binding repeat homology and LIM domains. There are three isoforms of this protein designated as α, β, γ; approximate molecular weight 68 kD. Paxillin is localized to actin-membrane attachment at sites of cell adhesion to extracellular matrix (focal adhesion). This protein undergoes cell adhesion-dependent tyrosine phosphorylation. Paxillin has been shown to interact with FAK, vinculin, src, Crk, p210BRC / ABL, talin, and integrin-β1. The Poly6007 antibody recognizes human, mouse, and rat paxillin and has shown to be useful for Western blotting.
1. Leventhal, P., et al., 1997. J. Biol. Chem. 272:5214. 2. Mazaki, Y., et al., 1997. J. Biol. Chem. 272:7437. 3. Kirchner, J., et al., 2003. J. Cell Sci. 116:975. 4. Tang, D., et al., 2003. J. Physiol. 42:858.
