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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 2-4 µl per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The monoclonal 1H9 antibody recognizes both mouse and human Pax5.
Application References:
1. Kallies A, et al. 2007. Immunity 26:555. (WB)
Daudi cell lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-Pax5 (clone 1H9). Proteins were visualized using a goat anti-rat IgG secondary conjugated to HRP and chemiluminescence detection.
Pax5, also known as BSAP (B cell specific activator protein), is a member of the paired box (pax) family of transcription factors. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. Pax5 is the only member of the pax family of transcription factors that is expressed in hematopoietic cells. During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development at the time of B cell commitment and is maintained throughout most subsequent stages. Suppression of Pax5 is essential for expression of Blimp-1 and the terminal differentiation of plasma cells. In the spleen, expression of Pax5 is higher in marginal zone B cells (B220+ CD21high CD23low) than in other B cells, especially the transition 1 stage (B220+ CD21- CD23-). In addition to its role in B cell development, Pax5 also affects VH-DJH heavy chain recombination as well as influencing the expression of many other B and non-B cell related proteins. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.
391 amino acids with predicted molecular weight of 42kD.
Distribution:
Nucleus
Function:
Play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
Interaction:
Interacts with DAXX. Binds DNA as a monomer. Binds TLE4.
Antigen References:
1. Liao F, et al. 1992. J. Immunol. 148:2909. 2. Nutt SL, et al. 1997. Genes Dev. 11:476. 3. Nera KP, et al. 2006. Immunity 24:283. 4. Fuxa M, et al. 2007. Immunity 178:3031.
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Purified anti-Pax-5
Daudi cell lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-Pax5 (clone 1H9). Proteins were visualized using a goat anti-rat IgG secondary conjugated to HRP and chemiluminescence detection.
