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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
This antibody is not suitable for immunoprecipitation.
HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-p90 Rsk antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. In addition to p90 Rsk, this antibody recognizes a non-specific protein with a molecular weight of approximately 66 kD.
p90 Rsk (ribosomal protein S6 kinase) is a 90 serine/threonine kinase and a member of the S6 kinase subfamily. This kinase is normally cytosolic but is transiently targeted to the membrane after activation. p90 Rsk phosphorylates a range of substrates including ribosomal protein S6 and is activated by the extracellular signal kinases ERK 1/2, PDK-1,and MAPK kinase cascade. p90 Rsk is phosphorylated and can interact with 14-3-3 β, and ERK 1/2 under quiescent conditions p90 Rsk is decreased upon mitogen stimulation. The Poly6048 antibody recognizes the N-terminal region of human p90 Rsk and has been shown to be useful for Western blotting.
Serine/Threonine family of kinases, S6 kinase subfamily; 90 kD
Distribution:
Cytosolic and transient targeting to membrane after activation
Function:
Phosphorylates range of substrates including ribosomal protein S6. Activated by extracellular signal kinases ERK 1/2, PDK-1, MAPK kinase cascade
Modification:
Phosphorylation
Interaction:
14-3-3 β, ERK 1/2 under quiescent conditions, decreased upon mitogen stimulation
Antigen References:
1. Chrestensen C, et al. 2002. J. Biol. Chem. 277:27733. 2. Moller D, et al. 1994. Am. J. Physiol. 266 C351. 3. Franklin R, et al. 1995. J. Immunol. 154:4965. 4. Cavet M, et al. 2003. J. Biol. Chem. 278:18376.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-p90 Rsk
HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-p90 Rsk antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. In addition to p90 Rsk, this antibody recognizes a non-specific protein with a molecular weight of approximately 66 kD.
