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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Non-transfected 293 cell lysates (Lane 1) and TAp63 alpha-transfected 293 cell lysates (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-p63 (TA) antibody (Poly6189). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
p63 (TA) is a member of the p53 family. This protein has multiple isoforms TA p63-α, -β, -γ, and δN p63-α, -β, -γ. The molecular weight of the TA p63 isoforms α=77 kD, β=62 kD, and γ=56 kD. This nuclear protein is essential for limb formation, epidermal morphogenesis, epithelial stem cell regeneration; δN isoforms are antagonistic to p53; TA isoforms can transactivate p53 targets. The p63 δN isoforms repress TA isoforms; mutant p53 abrogates p63 transactivation This protein interacts with p53, p73, and the various p63 TA- and δN isoforms. The Poly6189 antibody recognizes human p63 TA isoforms and has been shown to be useful for Western blotting.
Other Names:
p63, Isoforms TA p63-α, -β, -γ
Structure:
p53 family; six isoforms, TA p63α, β, γ, approximately 77 kD, 62 kD, 56 kD
Distribution:
Nuclear
Function:
Essential for limb formation, epidermal morphogenesis, epithelial stem cell regeneration; ΔN isoforms antagonistic to p53; TA isoforms can transactivate p53 targets
Regulation:
ΔN isoforms repress TA isoforms, mutant p53 abrogates p63 transactivation
Interaction:
p53; p73; TA- and ΔN isoforms
Antigen References:
1. Yang A, et al. 1998. Mol Cell. 2:305. 2. Celli J, et al. 1999. Cell. 99:143. 3. Ghioni P, et al. 2002. Mol Cell Biol. 22:8659. 4. Waltermann A, et al. 2003. Oncogene. 22:5686.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-p63 (TA)
Non-transfected 293 cell lysates (Lane 1) and TAp63 alpha-transfected 293 cell lysates (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-p63 (TA) antibody (Poly6189). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
